[MUSIC] Hello everyone, so welcome to our final module, module 6. And will be considering in this module, high throughput methods to screen for biological binders and inhibitors. And of course this has become a very important aspect of chemical biology and medicinal chemistry. And it feeds into several aspects that we've talked about, such as protein modification. And also a general theme of the course, which has been sort of drug design and application of chemical biology and biological phenomena. So if we think a little bit about how we got here, we'll look back at what has been a very interesting journey, I hope. We started off with an introduction. We tried to accommodate and adapt you to thinking about multiple different areas simultaneously. Putting together the concepts of chemistry, physics, and biology, to make something that was bigger than those three constitutive parts. Having done that, we brought you through a particular discussion about fluorescence, which we called the spark of life. And we talked about how florescence has become a common language that biologists use to express specific phenomena, and convert it just to the emission of light. We talked about how we can look at particular protein expression, localization. We can look at kinetics from a protein association. We also talked about mechanosensitivity of dyes through fluorescence lifetime imaging experiments. And we also looked at particular surface phenomenon through total internal fluorescence reflection microscopy, which is a very important technique. We then moved on to look at protein design and deliver. This was an important module looking at how we can build specific fusion proteins, and harness their powers to enable us to trigger signaling pathways, or perhaps my favorite, developer molecular nose. And then finally, we moved on into this couplet of modules, where we looked at the more the merrier. We started off with looking at photo encaging, where we triggered biological phenomena just through shining light. We released lipids and specific organelles, and we also looked at high resolution methods to detect specific electrophile labeling events of specific proteins. And then we fed that back into T-Rex that we've seen in module 4. So now, what we're going to do is, we're going to go even bigger. We're going to screen thousands, maybe even hundreds of thousands of molecules, and we'll show you how you can keep precise tabs on every single one of those molecules. In vitro, we're going to show you peptide nucleic acid encoded libraries. These are libraries of molecules that are made synthetically in the lab, but each one carries a specific barcode, just like your DNA and my DNA is different. These molecules have a similar descriptor attached to them, and we can therefore pan them against any protein we want to find the specific binder. In cells, we'll look at pathways signaling, and how that can be measured using just the emission of light. This time it won't be fluorescence light, it will be light that we generate bioluminescently using a specific protein, luciferase. And that will be a gauge for how much signaling is happening down a specific pathway. And using that, we'll screen for inhibitors of an important immuno-regulatory pathway, the sting pathway. And of course, like we wanted to do always, we will take you into one of the labs, and you can see peptide nucleic acid being used in action. And we'll talk to Luke, who is a specific chemist who works in the Winssinger lab. And professor Winssinger will talk to you specifically about protein nucleic acid in the first series of lectures here, and I will deal with the cell based screens later on. So we've been very happy to have you in this course, and we hope you like our final module. We feel that you should have derived from this course a love and appreciation, for how bringing new ideas together can really make something that is much bigger than anyone of us could possibly think of. We've got ideas and concepts here for many different people, and we've talked to over ten different people during this course, and they all have different opinions. They will all approach a problem differently, and a combination of those approaches is probably all that can be able to be leveraged to solve some of the most complex problems that exist today. So we have equipped you with an understanding of Interdisciplinary Science, which we hope you can apply generally. So thank you for joining the course, and we hope you enjoy these final modules. [MUSIC]
